Example 7: Spatial registration between two STARmap PLUS hippocampus sections.

• Run INSPIRE on STARmap PLUS sections from mouse hippocampus
  • Import packages
  • Load data
  • Data preprocessing
  • Build spatial graph
  • Run INSPIRE model
  • Access spot representations, proportions of spatial factors in spots, and gene loading matrix
  • Spatial distributions of spatial factors in tissues
  • Spot representations
  • Save results
• Spatially register the two STARmap PLUS sections using rigid transformation with MNNs
  • Load results
  • Register section 1 onto section 2
  • Registration result visualization
• Spatially register the two STARmap PLUS sections using rigid transformation with the ICP algorithm
  • Load results
  • Step 1: Register section 1 onto section 2 using rigid transformation with MNNs
  • Step 2: Register section 1 onto section 2 with the ICP algorithm guided by landmark spatial factors
  • Registration result visualization